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luna universal probe  (New England Biolabs)


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    Structured Review

    New England Biolabs luna universal probe
    Luna Universal Probe, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luna+universal+probe/Luna+Universal+Probe+One-Step+RT-qPCR+Kit/pm42811284-57-20-26
    Average 99 stars, based on 673 article reviews
    luna universal probe - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: A tiled amplicon protocol for culture-free whole-genome sequencing of M. tuberculosis from clinical specimens
    Article Snippet: .. RT-qPCR was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s recommendations for reaction composition (NEB): 10 μL Luna Universal Probe qPCR Master Mix, 0.8 μL forward primer (10 μM), 0.8 μL reverse primer (10 μM), and 0.4 μL probe; with 3 μL nuclease-free water and 5 μL template DNA, for a total reaction volume of 20 μL. ..

    Article Title: Evolutionary history of Jamestown Canyon virus reveals complex multi-vector ecology
    Article Snippet: 70 While extracted samples were provided from New York, Massachusetts, Michigan, and Pennsylvania, we performed our own nucleic acid extraction on all samples from Connecticut using the MagMAX viral/pathogen nucleic acid isolation kit (Thermo Fisher Scientific) and the Thermo Fisher protocol with reduced extraction volumes (100 uL). .. To assess samples for virus concentration, we employed a probe-based qPCR assay using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs). ..

    Article Title: Integrated validation of internally controlled one-step multiplex real-time RT-PCR for dengue virus serotyping and monoplex assay for quantification.
    Article Snippet: RNA was extracted from plasma or serum samples of patients with suspected dengue infection using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche, Cat. No. 06 543 588 001) on the MagNA Pure 96 instrument. .. The one-step multiplex real-time RT-PCR assay was performed using the Luna® Universal Probe One-Step RT-qPCR Kit (NEB, E3006L) with 5 μL RNA in a 20 μL reaction volume, including 0.2 μM MgCl2. ..

    Article Title: Highly pathogenic avian influenza A (H5N1) in a backyard flock in Serbia: implications of low biosecurity
    Article Snippet: Extraction of viral RNA from the obtained supernatant was performed with the IndiSpin Pathogen Kit (Indical Bioscience GmbH, Germany). .. The influenza A virus genome was detected using the Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, England) with previously published primers and probes ( ). ..

    Article Title: Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growth
    Article Snippet: RNA was isolated using the MagMAX Prime Viral/Pathogen NA Isolation Kit (ThermoFischer, cat # A58145) and a KingFischer 711 Automated Extraction and Purification System (Thermo). .. To determine the viral copy number of collected supernatant samples, we used a 6-carboxy-fluorescein (FAM)-labeled TaqMan probe (ThermoFisher) that targets the E gene and the Luna Universal Probe One-Step RT-qPCR kit (NEB, cat #E3006S). ..

    Article Title: Epigenetic dysregulation of IRF9 drives excessive interferon signaling in COPD.
    Article Snippet: Amplicons were phosphorylated with T4 PNK (NEB) and ligated with TruSeq adapters using T4 DNA ligase (NEB) (Glenn et al, 2019). .. Dual-indexing was carried out with the TruSeq panel of indexing primers in a short-cycle PCR using Luna Universal Probe One-Step RT-qPCR reagents (NEB). .. The pooled adapter libraries were purified and size-selected using AMPure XP beads (Beckman Coulter) or carboxyl-coated BOMB beads (Oberacker et al, 2019), and quantified with Qubit Fluorometer using dsDNA-HS reagents (Invitrogen).

    Article Title: Detection of Influenza D Virus using Reverse Transcription Loop-Mediated Isothermal Amplification.
    Article Snippet: .. We performed the reactions in a 384- well Quant Studio 5 Real-Time PCR System (Thermo Fisher Scientific, https://www.thermofisher.com) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), according to the manufacturer’s instructions. ..

    Article Title: γδ T cell knockout chickens as a model to investigate the T cell response against Infectious Bursal Disease infection.
    Article Snippet: .. The mRNA-expression of IFN-γ (Bagheri et al., 2022), GZMA (Lee et al., 2015) and FoxP3 (Burkhardt et al., 2022) was measured using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, Inc., Ipswich, MA, USA) and the QuantStudio 3 system (Thermo Fisher Scientific Inc., Waltham, MA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: A tiled amplicon protocol for culture-free whole-genome sequencing of M. tuberculosis from clinical specimens
    Article Snippet: .. RT-qPCR was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s recommendations for reaction composition (NEB): 10 μL Luna Universal Probe qPCR Master Mix, 0.8 μL forward primer (10 μM), 0.8 μL reverse primer (10 μM), and 0.4 μL probe; with 3 μL nuclease-free water and 5 μL template DNA, for a total reaction volume of 20 μL. ..

    Article Title: Evolutionary history of Jamestown Canyon virus reveals complex multi-vector ecology
    Article Snippet: 70 While extracted samples were provided from New York, Massachusetts, Michigan, and Pennsylvania, we performed our own nucleic acid extraction on all samples from Connecticut using the MagMAX viral/pathogen nucleic acid isolation kit (Thermo Fisher Scientific) and the Thermo Fisher protocol with reduced extraction volumes (100 uL). .. To assess samples for virus concentration, we employed a probe-based qPCR assay using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs). ..

    Article Title: Detection of Influenza D Virus using Reverse Transcription Loop-Mediated Isothermal Amplification.
    Article Snippet: .. We performed the reactions in a 384- well Quant Studio 5 Real-Time PCR System (Thermo Fisher Scientific, https://www.thermofisher.com) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), according to the manufacturer’s instructions. ..

    Virus:

    Article Title: Evolutionary history of Jamestown Canyon virus reveals complex multi-vector ecology
    Article Snippet: 70 While extracted samples were provided from New York, Massachusetts, Michigan, and Pennsylvania, we performed our own nucleic acid extraction on all samples from Connecticut using the MagMAX viral/pathogen nucleic acid isolation kit (Thermo Fisher Scientific) and the Thermo Fisher protocol with reduced extraction volumes (100 uL). .. To assess samples for virus concentration, we employed a probe-based qPCR assay using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs). ..

    Article Title: Highly pathogenic avian influenza A (H5N1) in a backyard flock in Serbia: implications of low biosecurity
    Article Snippet: Extraction of viral RNA from the obtained supernatant was performed with the IndiSpin Pathogen Kit (Indical Bioscience GmbH, Germany). .. The influenza A virus genome was detected using the Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, England) with previously published primers and probes ( ). ..

    Concentration Assay:

    Article Title: Evolutionary history of Jamestown Canyon virus reveals complex multi-vector ecology
    Article Snippet: 70 While extracted samples were provided from New York, Massachusetts, Michigan, and Pennsylvania, we performed our own nucleic acid extraction on all samples from Connecticut using the MagMAX viral/pathogen nucleic acid isolation kit (Thermo Fisher Scientific) and the Thermo Fisher protocol with reduced extraction volumes (100 uL). .. To assess samples for virus concentration, we employed a probe-based qPCR assay using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs). ..

    Multiplex Assay:

    Article Title: Integrated validation of internally controlled one-step multiplex real-time RT-PCR for dengue virus serotyping and monoplex assay for quantification.
    Article Snippet: RNA was extracted from plasma or serum samples of patients with suspected dengue infection using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche, Cat. No. 06 543 588 001) on the MagNA Pure 96 instrument. .. The one-step multiplex real-time RT-PCR assay was performed using the Luna® Universal Probe One-Step RT-qPCR Kit (NEB, E3006L) with 5 μL RNA in a 20 μL reaction volume, including 0.2 μM MgCl2. ..

    Polymerase Chain Reaction:

    Article Title: Epigenetic dysregulation of IRF9 drives excessive interferon signaling in COPD.
    Article Snippet: Amplicons were phosphorylated with T4 PNK (NEB) and ligated with TruSeq adapters using T4 DNA ligase (NEB) (Glenn et al, 2019). .. Dual-indexing was carried out with the TruSeq panel of indexing primers in a short-cycle PCR using Luna Universal Probe One-Step RT-qPCR reagents (NEB). .. The pooled adapter libraries were purified and size-selected using AMPure XP beads (Beckman Coulter) or carboxyl-coated BOMB beads (Oberacker et al, 2019), and quantified with Qubit Fluorometer using dsDNA-HS reagents (Invitrogen).



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    Image Search Results


    List of reagents and buffers. BSA, bovine serum albumin; CCK-8, cell counting kit-8; DAPI, 4′,6-diamidino-2-phenylindole; DMEM, Dulbecco’s modified Eagle’s medium; DMSO, dimethyl sulfoxide; DPBS, Dulbecco’s phosphate-buffered saline; dNTP, deoxynucleotide triphosphate; FBS, fetal bovine serum; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HBSS, Hanks’ balanced salt solution; MEM, minimum essential medium; P/S, penicillin/streptomycin; RNAse, ribonuclease.

    Journal: Science Advances

    Article Title: Airway epithelia clear rhinovirus infection via two waves of cell extrusion

    doi: 10.1126/sciadv.aef2562

    Figure Lengend Snippet: List of reagents and buffers. BSA, bovine serum albumin; CCK-8, cell counting kit-8; DAPI, 4′,6-diamidino-2-phenylindole; DMEM, Dulbecco’s modified Eagle’s medium; DMSO, dimethyl sulfoxide; DPBS, Dulbecco’s phosphate-buffered saline; dNTP, deoxynucleotide triphosphate; FBS, fetal bovine serum; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HBSS, Hanks’ balanced salt solution; MEM, minimum essential medium; P/S, penicillin/streptomycin; RNAse, ribonuclease.

    Article Snippet: M3004E , , New England Biolabs , .

    Techniques: CCK-8 Assay, Modification, Saline, Cell Culture, Cell-Signaling, Concentration Assay